The LM were cultivated， isolated and identified from the patient’s cerebrospinal fluid， and bacterial De novo sequencing was conducted by the Illumina HiSeq 3000 system to obtain the whole genome data of the strain.Furthermore， the evolutionary tree of the LM was constructed and the drug-resistant related genes were identified by gene assembly， gene prediction， gene annotation， single nucleotide polymorphism（SNP） analysis， and phylogenetic analysis and other bioinformatics methods.

The bacteria in the cerebrospinal fluid were isolated and cultured， and identified as LM by mass spectrometry.The size of the assembled bacterial genome after sequencing was 3 008 507 bp.By aligned with database， its genome was accordance with 88% of LM genome， confirming that the bacteria was LM.The obtained listeria strains were close to FSL N1-017 and SLCC2540 by aligning 38 representative listeria genome construction phylogenetic trees in the NCBI database，and they had no evolutionary relationships with human-derived LM that were reported worldwide. It was considered to be a newly discovered clinically infectious LM in China.The drug resistance analysis revealed that the obtained LM genome contained some drug-resistant genes associated with the major facilitator superfamily（MFS）transporters，and four of them were matched the drug resistance genes in the database EDG-e strain transporter annotation， suggesting that the strain had strong clinical resistance.

The cultured bacteria from cerebrospinal fluid of clinical meningitis patient is identified as a new type of clinically infectious pathogenic LM strain with strong clinical drug resistance.