Abstract:

Objective To explore the expressions of nuclear factor(NF-κB ) and tumor necrosis factor(TNF-α) protein in  serum and carcinoma tissue of the pati
ents with cervical cancer,and to clarify their biological significance and the values in early combined diagnosis.Methods The blood samples were collected  and colposcope was performed in 100 patients with high-risk factors of cervical lesion (37 cases of cervicitis,39 cases of cervical intraepithlial neoplasia,24 cases of cervical cancer).The ELISA method was used to detect the contents of NF-κB and TNF-α protein in the serum and cervix  tissue.The expression levels of NF-κB and TNF-α mRNA in cervix tissue were determined with RT-PCR assa.The immunohistochemical method was used to detect the expressions of NF-κB and TNF-α protein in cervix tissue;the relationship with clinical and pathological features was analyzed.Results The contents of NF-κB and TNF-α protein in serum and cervix tissue in the patients with cervical intraepithelial neoplasia and cervical cancer were significantly higher than those in the patients with cervicitis(P<0.01).The contents of NF-κB and TNF-α protein in the patients with cervical cancer were significantly higher than those in the patients with cervical intraepithelial neoplasia(P<0.01).The NF-κB and TNF-α  mRNA expression levels and  protein levels in the cervix tissue of the patients with cervicitis were  significantly lower than those of the patients with cervical neoplasia and cervical cancer(P<0.01),and the expression levelsNF-κB and TNF-α mRNA  and NF-κB and TNF-α protein  in cervical tissue of the patients with cervical intraepithelial neoplasia were significantly lower than those of the patients with cervical cancer(P<0.01).The expressions of mRNA and protein of NF-κB and TNF-α showed positive correlation between  the patients with cervicitis,cervical intraepithelial neoplasia and cervical cancer(r=0.69，P=0.037).Conclusion NF-κB and TNF-α can be independent as markers for early diagnosis of cervical precancerous lesions and cervical cancer,and the positive correlation between them can make the combined detection to improve the early diagnosis rate of cervical cancer.

Key words: cervix neoplasms, cervical precancerous lesions, nuclear factor, tumor necrosis factor