Abstract:

Objective To investigate the expressions of TPX2 and Aurora A  in oral squamous cell carcinoma(OSCC)tissue and explore the significances of TPX2 and Aurora A expressions in the  treatment and prognosis of oral squamous cell carcinoma.Methods 61 cases of oral squamous cell carcinoma were chosen as experimental group, other 29cases of adjacent tissue of cancer and 61 cases of normal oral mucosa tissue were chosen as control group.　Immunohistochemical staining  was used to detect  the positive expressions  of TPX2 and Aurora A.The percentages of positive cells were observed under  microscope.The correlation between TPX2 and Aurora A protein expressions was analyzed with Spearman analysis.Results The results of immunohistochemical staining showed that the positive expression rates of TPX2 protein in normal mucosa tissue,adjacent tissue of cancer and cancer tissue were 4.9% (3/ 61),51.7% (15/29),and 86.9% (53/61);and there were statistically significant difference among all groups (P<0.05).The expression of TPX2 protein was closely related to the lymph node metastasis of oral squamous cell carcinoma (P<0.05).The positive expression rates of Aurora A protein in normal mucosa tissue,adjacent tissue of cancer and cancer tissue were  3.3% (2/ 61),44.8% (13/29),and 72.1% (44/61);and there were statistically significant difference among all groups (P<0.05).And with the increasing of Aurora A expression level,the malignant  degrees and possibility of lymph node metastasis in oral squamous cell carcinomas were also increased,which had statistically significant difference among groups (P<0.05).The TPX2 protein expression in oral squamous cell carcinoma tissue had positive correlation with the Aurora A protein expression(r=0.445，P<0.01).Conclusion TPX2 and Aurora A may play important roles in promoting the infiltration and metastasis of mucosal epithelium in oral squamous cell carcinoma,and the relationship between the two genes is collabortion and mutual promotion.

Key words: oral squamous cell carcinoma, immunohis
tochemistry, targeting protein for Xenopus kinesin-like protein 2