Abstract:

Abstract:Objective  To observe the effect of Mongolian medicine Tonglaga-5 Pill on the osteoporosis induced by retinoic acid in the rats,and to provide experminted basis for its clinical application.Methods 60 female Wistar rats were randomly divided into blank group,model group,Ossptide Tablets group,Tonglaga-5 Pill groups with low,medium and high doses (n=10).The osteoporosis models were induced by retinoic acid in the rats.The levels of serum calcium (Ca),phosphorus (P),alkaline phosphatase (ALP),transforming growth factor β1 (TGF-β1),insulin like growth factor (IGF-1) and bone indexes were detected and the contents of Ca and P in ash were also detected,and the changes of bone histomorphometry of the rats were observed under light microscope by HE staining.Results Compared with model group,the serum calcium and   P contents of the rats in Tonglaga-5 Pill groups were increased,but there were no significant differences(P>0.05);the ALP levels were  decreased significantly(P<0.05);the TGF-β1 and IGF-1 had no significant changes(P>0.05);the contents of calcium and P in  ash were increased in different degrees,but there were no significant differences (P>0.05).Compared with control group,the femur maximum width index and minmum width index of the rats in model group were significantly decreased(P<0.05 or P<0.01)；the femur length index was increased(P<0.05).Compared with model group,the femur maximum width index and minmum width index of the rats in Tonglaga-5 Pill groups had no changes(P>0.05).Under light microscope,the rich and full bone trabecula was found in the rat femur in control group;the bone trabecula in model group was decreased,and the bone trabecula in Tonglaga-5 Pill groups was increased.Compared with model group,the trabecular bone areas in Tonglage-5 pill groups and Ossotide group were increased(P<0.05).Conclusion  Tonglaga-5 Pill  displays a certain preventive effect on  the rats with retinoic acid-induced osteoporosis,which can blocks the bone mineral loss,bone tissue structure changes and bone quality decreasing   effectively.

Key words: Tonglaga-5 Pill, osteoporosis, retinoic acid, rats, wistar