急性髓系白血病患者外周血外泌体中miR-4286表达水平检测及其诊断价值

doi:10.13481/j.1671-587X.20230222

Abstract

Abstract:

Objective To detect the expression of miR-4286 in exosome in peripheral blood of the patients with acute myeloid leukemia（AML）， and to clarify the value of miR-4286 in the diagnosis of AML. Methods The peripheral blood samples from 45 newly diagnosed patients with AML （AML group） and 35 concurrent healthy subjects（control group） were collected.The exosomes from serum of the subjects in two groups were obtained by over speend contrifugation； the morphology of the centrifuged products was observed under transmission electron microscope（TEM）；and the particle diameter was detected by nanoparticle tracink analysis（NTA）； the expressions of surface markers CD63 and CD9 proteins were analyzed by Western blotting method；the expression levels of miR-4286 in exosome of the subjects in two groups were detected by real-time fluorescence quantitative PCR（RT-qPCR） method；the receiver operating characteristic （ROC） curve was used to calculate the area under curve （AUC）； the sensitivity and specificity of miR-4286 in the diagnosis of AML were analyzed. Results The TEM results showed that the extracted exosomes in peripheral blood showed a membrane structure with roundness in apperance and concave in the middle， and the diameter was about 30－100 nm；the NTA results showed that the particle sizes of exosomes were mainly distributed at 30－150 nm；the Western blotting results showed that the exosomes all expressed CD63 and CD9 proteins，with the typical characteristics of the exosomes.The RT-qPCR results showed that the expression level of exosome miR-4286 in peripheral blood of the patients in AML group was significantly higher than that in control group（Z=－5.543，P<0.01）. The ROC curve analysis showed that when the critical value of expression level of miR-4286 in exosome was 3.182， the AUC was 0.862 9（95%CI：0.774 7，0.937 1， P<0.01）， the sensitivity was 66.67%，the specificity was 97.14%，and the diagnostic efficacy was good. Conclusion The miR-4286 in exosome in peripheral blood has high diagnostic efficiency for AML；it can be used as a diagnostic marker for AML， and has high clinical diagnostic value.

Key words: Acute myeloid leukemia, Exosome, microRNA-4286, Diagnostic efficacy, Biomarker

CLC Number:

R446.11

Haiwei QUAN,Yali ZHANG,Han WANG,Yijiu AI,Yutong ZOU,Wei XIA. Detection of expression level of miR-4286 in exosome in peripheral blood of patients with acute myeloid leukemia and its diagnostic value[J].Journal of Jilin University(Medicine Edition), 2023, 49(2): 460-466.

Figures/Tables 5

References 34

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Detection of expression level of miR-4286 in exosome in peripheral blood of patients with acute myeloid leukemia and its diagnostic value

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