Abstract:

Objective To discuss the effect of ganoderic acid A （GAA） on the proliferation， apoptosis， and migration ability of the PC-9 cells， and to clarify its mechanism. Methods The non-small cell lung cancer （NSCLC） PC-9 cells were cultured in vitro and divided into control group （without GAA）， low dose of GAA group （0.1 mmol·L^－1 GAA）， and high dose of GAA group （0.5 mmol·L^－1 GAA）. MTT method was used to detect the proliferation rates of the PC-9 cells in various groups； flow cytometry was used to detect the apoptotic rates of the PC-9 cells in various groups； scratch wound healing assay was used to detect the scratch healing rates of the PC-9 cells in various groups； Transwell chamber assay was used to detect the migration abilities of PC-9 cells in various groups； the expression levels of vascular endothelial growth factor （VEGF） and hypoxia-inducible factor-1α （HIF-1α） mRNA and protein in the PC9 cells in various groups were detected by real-time fluorescence quantitative PCR （RT-qPCR） and Western blotting methods. Results The MTT assay results showed that compared with control group， the proliferation rate of the cells in low dose of GAA group was decreased after treated for 48 and 72 h （P<0.05）； after treated for 24， 48， and 72 h， the proliferation rate of the cells in high dose of GAA group was decreased （P<0.05）； compared with low dose of GAA group， the proliferation rate of the cells in high dose of GAA group was decreased after treated for 24， 48， and 72 h （P<0.05）.The flow cytometry results showed that compared with control group and low dose of GAA group， the apoptotic rate of the cells in high dose of GAA group was increased （P<0.05）. The cell scratch wound healing assay results showed that compared with control group and low dose of GAA group， the scratch wound healing rate of the cells in high dose of GAA group was decreased （P<0.05）. The Transwell chamber assay results showed that compared with control group and low dose of GAA group， the number of migration cells in high dose of GAA group was decreased （P<0.05）. The RT-qPCR and Western blotting results showed that compared with control group and low dose of GAA group， the expression levels of HIF-1α and VEGF mRNA and proteins in the cells in high dose of GAA group were decreased （P<0.05）. Conclusion High dose of GAA can inhibit the proliferation of the PC-9 cells and promote the apoptosis， and its mechanism may be related to regulating the expressions of VEGF and HIF-1α mRNA and proteins.

Key words: Ganoderic acid A, Cancer，non small cell lung, Vascular endothelial growth factor, Hypoxia-inducible factor-1α, Cell proliferation, Apoptosis