基于核酸适配体功能化石墨纳米颗粒荧光探针的17β-雌二醇快速检测方法

doi:10.13278/j.cnki.jjuese.20180036

Abstract

Abstract: In order to establish a simple, rapid and specific method for detecting 17β-estradiol in complex environmental water, we prepared a novel fluorescence probe by using graphite nanoparticles(GN) as excellent quencher, aptamers as recognition elements,and 1-pyrenebutyric acid N-hydroxysuccinimide ester as heterobifunctional crosslinking reagent. We studied the effects of initial concentration of aptamer on the construction of aptamer probe, the change of aptamer probe's concentration on fluorescence signals, and the optimal conditions for 17β-estradiol detection. The results showed that the aptamers were successfully anchored on the surface of GN, and the fluorescence aptamer probe was well synthesized. The optimum initial concentration of aptamer for the preparation of fluorescence probe was 1.0 μmol/L; the optimum concentration of the fluorescent probe for analysis was 4 μg/mL. Under the optimal condition, the relative fluorescence intensity was proportional to the concentration of 17β-estradiol ranging from 50 to 800 ng/mL, and the limit of detection was 34.5 ng/mL. This method is simple, quick and specific for the detection of 17β-estradiol.

Key words: graphite nanoparticle, aptamer, 17β-estradiol, probe, rapid detection

CLC Number:

X502

Piao Yunxian, Qi Xiaoli, Wang Xiang, Kang Boquan, Shi Yuxi, Hu hui, Yang Yuesuo. Rapid Detection of 17β-Estradiol Based on Fluorescent Probe of Functionalized Graphite Nanoparticle with Aptamers[J].Journal of Jilin University(Earth Science Edition), 2019, 49(4): 1137-1144.

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Rapid Detection of 17β-Estradiol Based on Fluorescent Probe of Functionalized Graphite Nanoparticle with Aptamers

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