Abstract: As to the available components in the municipal refuse, the ethanol is produced by choosing microzyme with the refuse liquid hydrolyzed by enzyme. Through the orthogonal experiments, the optimum hydrolysis conditions are acquired as follows: pH is 5.5, 8 mg of cellulosic enzyme, 6 mg of amylase, 5 mL of water, 65℃ and the hydrolyzing time duration of 40 minutes. The fermenting experiments are carried out using microzyme named AQ selected as the best bacterium, and the effects of output are discussed by choosing inoculation, pH values and temperatures as the affecting factors. The experimental results show that the maximum output of ethanol is 148.37 mg per gram dry municipal refuse.

Key words: municipal refuse, hydrolysis, ethanol fermentation, ethanol