动性球菌产生胞外脂肪酶的条件

• • 上一篇

动性球菌产生胞外脂肪酶的条件

王海晶　梅子青　刘贤忠　黄宜兵　朴真三

吉林大学生命科学学院,长春,130023

收稿日期:2002-07-01 修回日期:1900-01-01
通讯作者: 朴真三

Conditions for Plancoccus Migula Yielding Lipase in Vitro

WANG Hai-jing , MEI Zi-qing, LIU Xian-zhong , HUANG Yi-bing , PIAO Zhen-san

College of Life Science , Jilin University , Changchun 130023 , China

Received:2002-07-01 Revised:1900-01-01
Contact: PIAO Zhen-san

摘要/Abstract

摘要： 测定了动性球菌产生胞外脂肪酶的酶活力,对动性球菌产生胞外脂肪酶的条件进行了研究.结果表明,当碳源为葡萄糖,氮源为蛋白胨,初始pH值为6.0时脂肪酶产率最高;K2HPO4,KH2PO4,CaCl2,ZnSO4和NaCl对脂肪酶产率有促进作用;CuSO4和MgSO4对脂肪酶产率有一定影响;FeCl3,BaCl2,CoSO4和MnSO4对脂肪酶产率影响较大;非离子表面活性剂吐温和聚乙烯醇均能促进脂肪酶的生成,且吐温比聚乙烯醇效果更好.

关键词: 动性球菌, 胞外脂肪酶, 酶活力

Abstract: We mensurated the lipase activity of Plancoccus Migula fermentation liquid , and studied the effects of all kinds of growth elements on the lipase yield. The result shows that the optimum conditions to yield lipase are that carbon source is glucose ; nitrogen source is peptone ; initial pH is 6.0 , and while K²HPO⁴, KH²PO⁴ ,CaCl² , ZnSO⁴ and NaCl can promote lipase production ; CuSO⁴ and MgSO⁴ can partly decrease lipase output ; FeCl³ , BaCl² , CoSO⁴ and MnSO⁴ inhibit lipase production completely. In addition non-ionic surface-active agents for instance Tween also promote lipase production.

Key words: plancoccus migula, lipase in vitro, enzyme activity

中图分类号:

X172

王海晶　梅子青　刘贤忠　黄宜兵　朴真三. 动性球菌产生胞外脂肪酶的条件[J]. J4, 2002, 40(04): 430-432.

WANG Hai-jing , MEI Zi-qing, LIU Xian-zhong , HUANG Yi-bing , PIAO Zhen-san. Conditions for Plancoccus Migula Yielding Lipase in Vitro[J]. J4, 2002, 40(04): 430-432.

[1]	安佰义, 郑璐, 吴卓夫, 王磊, 王智, 陈光. 超声预处理对酶促拆分布洛芬反应的影响[J]. 吉林大学学报(理学版), 2016, 54(05): 1177-1180.
[2]	王海晶，梅子青，朴真三. 动性球菌代谢变压器绝缘油及其代谢产物对绝缘油介损的影响[J]. J4, 2004, 42(02): 293-297.