细胞粘附位点RGD三肽脂质体的制备及表征

细胞粘附位点RGD三肽脂质体的制备及表征

杨彤，周云，王娜，徐力, 吴晓霞，张学忠

吉林大学分子酶学工程教育部重点实验室, 长春 130023

收稿日期:2002-11-26 修回日期:1900-01-01 出版日期:2003-07-26 发布日期:2003-07-26
通讯作者: 张学忠

Preparation and Characterization of Liposome of RGD Tripeptide as Cell Recognition Site

YANG Tong, ZHOU Yun-yun, WANG Na, XU Li, WU Xiao-xia, ZHANG Xue-zhong

Key Laboratory of Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130023, China

Received:2002-11-26 Revised:1900-01-01 Online:2003-07-26 Published:2003-07-26
Contact: ZHANG Xue-zhong

摘要/Abstract

摘要： 对RGD（Arg-Gly-Asp）三肽脂质体载药剂型进行研究，确定了薄膜超声法制备脂质体的路线. 电子显微镜观察表明，脂质体粒径均匀，为100 nm右，属于小单室脂质体. 脂质体中RGD量为2.4~2.5 mg/mL，包封率达到70%. 脂质体稳定性较好，而且具备pH 5.4~6.0的靶向性，可能成为具有较好肿瘤细胞靶向性的脂质体制剂.

关键词: RGD, 抗肿瘤转移, 脂质体, 靶向药物

Abstract: In this paper, RGD(Arg-Gly-Asp) liposome formulation as a drug carrier was conducted. A film-ultrasonic technique was chosen for pr eparation of liposome. The observation by electron microscope indicated that the particle diameter of liposome was basically homogenous, being about 100 nm and small vesicles. The content of RGD in liposome is 2.4～2.5 mg/mL with the entrapment efficiency of 70%. RGD liposome prepared by the method used has not only better stability, but also target tropism at pH 5.4～6.0. Therefore, the prepared liposome here may become a kind of RGD formulation with better target tropism to tumor.

Key words: RGD, inhibition of tumor metastasis, liposome, targeted delivery

中图分类号:

Q503

杨彤，周云，王娜，徐力, 吴晓霞，张学忠. 细胞粘附位点RGD三肽脂质体的制备及表征[J]. J4, 2003, 41(03): 356-360.

YANG Tong, ZHOU Yun-yun, WANG Na, XU Li, WU Xiao-xia, ZHANG Xue-zhong. Preparation and Characterization of Liposome of RGD Tripeptide as Cell Recognition Site[J]. J4, 2003, 41(03): 356-360.

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