乙酰肉碱的化学合成与酶法拆分

乙酰肉碱的化学合成与酶法拆分

丁天兵¹，王智¹，翁良¹，于大海¹ ，郑良玉¹，曹淑桂¹, 苟小军²

1. 吉林大学分子酶学工程教育部重点实验室，长春 130023; 2. 成都大学生物工程系，成都 610106

收稿日期:2003-01-06 修回日期:1900-01-01 出版日期:2003-07-26 发布日期:2003-07-26
通讯作者: 曹淑桂

Chemical Synthesis of Acetyl-L-carnitine and Its Enzymatic Resolution

DING Tian-bing¹, WANG Zhi¹, WENG Liang¹, YU Da- hai¹, ZHENG Liang-yu¹, CAO Shu-gui¹, GOU Xiao-jun²

1. Key Laboratory for Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130023, China; 2. Department of Biolgical Engineering, Chengdu University, Chengdu 610106, China

Received:2003-01-06 Revised:1900-01-01 Online:2003-07-26 Published:2003-07-26
Contact: CAO Shu-gui

摘要/Abstract

摘要： 采用化学方法以肉碱为底物，合成了外消旋乙酰肉碱，精制后可获得纯度99%以上的乙酰肉碱，收率为96%. 利用蜗牛酶（Snail Enzyme）对其进行不对称酯水解反应，发现蜗牛酶对乙酰肉碱具有高度立体选择性，并且可得到光学纯度为98%的乙酰-L- 肉碱.

关键词: 乙酰肉碱, 化学合成, 蜗牛酶, 拆分

Abstract: Acetyl-L-carnitine was synthesized from racemic carnitine chloride and acetic acid by chemical means. The purity of the refined product was over 99% a nd the yield was about 96%. Acetyl-L-carnitine with 98% ee was obtained by Snail enzyme catalyzing the resolution of racemic acetyl carnitine.

Key words: acetyl-carnitine, chemical synthesis, Snail enzyme, resolution

中图分类号:

丁天兵，王智，翁良，于大海，郑良玉，曹淑桂, 苟小军. 乙酰肉碱的化学合成与酶法拆分[J]. J4, 2003, 41(03): 399-401.

DING Tian-bing, WANG Zhi, WENG Liang, YU Da- hai, ZHENG Liang-yu, CAO Shu-gui, GOU Xiao-jun. Chemical Synthesis of Acetyl-L-carnitine and Its Enzymatic Resolution[J]. J4, 2003, 41(03): 399-401.

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