仿水溶剂促进酶促拆分环氧丙醇的研究

仿水溶剂促进酶促拆分环氧丙醇的研究

王智¹，苟小军²，于大海¹，郑良玉¹，张桂荣¹，曹淑桂¹

1. 吉林大学分子酶学工程教育部重点实验室，长春 130023； 2. 成都大学生物工程系分子进化工程实验室，成都 610106

收稿日期:2003-06-26 修回日期:1900-01-01 出版日期:2004-01-26 发布日期:2004-01-26
通讯作者: 曹淑桂

Enzymatic resolution of glycidol improved by water-mimicking solvent

WANG Zhi¹, GOU Xiao-jun², YU Da-hai¹, ZHENG Liang-yu¹, WENG Liang¹, ZHANG Gui-rong¹, CAO Shu-gui¹

1. Key Laboratory for Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130023, China; 2. Key Laboratory of Molecular Evolution and Engineering, Department of Biology Engineering, Chengdu University, Chengdu 610106, China

Received:2003-06-26 Revised:1900-01-01 Online:2004-01-26 Published:2004-01-26
Contact: CAO Shu-gui

摘要/Abstract

摘要： 利用脂肪酶在非水介质中对外消旋环氧丙醇进行不对称酯合成反应，重点研究了强极性有机溶剂作为仿水溶剂完全替代反应体系中的微量“必须”水对酶促拆分反应的影响. 实验结果表明, 乙腈作为仿水溶剂（最佳用量为0.4%）在适量无水硫酸钠（0.04 g左右）的配合下，可以有效除去酯合成反应产生的水. 在最适反应条件下，制备得到光学纯度约为90%的单一手性环氧丙醇酯.

关键词: 环氧丙醇, 脂肪酶, 酯合成反应, 仿水溶剂

Abstract: The asymmetric esterification of glycidol catalyzed by lipase was carried out in a nonaqueous medium. The effect of the replacement of water necessary for the funct ion of lipase with water-mimicking solvent on the resolution was investigated. It's found that the water produced from enzymatic esterification could be removed s uccessfully by addition of acetonitrile(0.4%, volume fraction) and 0.04　g anhydrous Na₂SO₄ into the reaction system. Under the optimal conditions, R- glycidyl butyrate with about ee 90% was prepared.

Key words: glycidol, lipase, esterification, water-mimicking solvent

中图分类号:

王智，苟小军，于大海，郑良玉，张桂荣，曹淑桂. 仿水溶剂促进酶促拆分环氧丙醇的研究[J]. J4, 2004, 42(01): 126-129.

WANG Zhi, GOU Xiao-jun, YU Da-hai, ZHENG Liang-yu, WENG Liang, ZHANG Gui-rong, CAO Shu-gui. Enzymatic resolution of glycidol improved by water-mimicking solvent[J]. J4, 2004, 42(01): 126-129.

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