血浆p16基因甲基化在原发性肝细胞癌诊断中的作用

血浆p16基因甲基化在原发性肝细胞癌诊断中的作用

罗速^1，2，张逢春¹，刘成柏³，王潇^{1, 庄江兴^3，4，张今²}

1．　北华大学医学院，吉林　132013； 2．　吉林大学酶学工程重点实验室，长春　1 30023；　3．　吉林大学生命科学学院，长春　130023； 4. 福建省漳州市药品检验所，漳州 363000

收稿日期:2003-12-05 修回日期:1900-01-01 出版日期:2004-04-26 发布日期:2004-04-26
通讯作者: 罗速

Methylation of p16 gene in plasma in diagnosis of primary hepatocellular carcinoma

LUO Su^1,2， ZHANG Feng-chun¹， LIU Cheng-bai³, WANG Xiao¹, ZHUANG Jiang-xing^3,4， ZHANG Jin²

1. Medical College of Beihua University, Jilin 132013, China;2. Key Laboratory of Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130023, China; 3. College of Life Science, Jilin University, Changchun 130023, China; 4. Fujian Zhangzhou Institute of Drug Control, Zhangzhou 363000, China

Received:2003-12-05 Revised:1900-01-01 Online:2004-04-26 Published:2004-04-26
Contact: LUO Su

摘要/Abstract

摘要： 用甲基化特异性-多聚酶链反应（MSP）方法，检测原发性肝细胞癌（HCC）、肝硬化、慢性肝炎病人及健康人各30例的血浆DNA甲基化的p16基因. 结果显示， HCC病人血浆p16 基因甲基化率为67%，慢性肝炎、肝硬化及健康者未发生甲基化.

关键词: 原发性肝细胞癌, p16基因, 甲基化

Abstract: Using the methylation specific PCR(MSP) to detect the methylation of p16 geng of each of 30 patients with primary hepatocellular carcinoma(HCC), 30 patients with cirrhosis, 30 patients with chronic hepatitis and 30 healthy individ uals, respectively. The results show that the methylation of p16 gene was foun ded in 20 of the 30 patients (67%) with primary hepatocellular carcinoma. In the other groups was without the methylation of p16 gene.

Key words: primary hepatocellular carcinoma, p16 gene, methylation

中图分类号:

Q953

罗速，张逢春，刘成柏，王潇, 庄江兴，张今. 血浆p16基因甲基化在原发性肝细胞癌诊断中的作用[J]. J4, 2004, 42(02): 298-301.

LUO Su， ZHANG Feng-chun， LIU Cheng-bai, WANG Xiao, ZHUANG Jiang-xing， ZHANG Jin. Methylation of p16 gene in plasma in diagnosis of primary hepatocellular carcinoma[J]. J4, 2004, 42(02): 298-301.

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