J4

• 环境科学 • 上一篇    下一篇

稠油降解菌的筛选及其生物表面活性剂的特性

伏亚萍1, 李鱼1,2, 王健1, 曹周礼3, 段勇1, 郭书海2   

  1. 1. 吉林大学 环境与资源学院, 长春 130012; 2. 中国科学院 沈阳应用生态研究所, 沈阳 110016; 3. 长春科技创业服务中心, 长春 130012
  • 收稿日期:2006-06-16 修回日期:1900-01-01 出版日期:2007-01-26 发布日期:2007-01-26
  • 通讯作者: 李鱼

Screening of Biosurfactantproducing Thick Oildegrading Bacteria and Its Characteristics of Biosurfactants

FU Yaping1, LI Yu1,2, WANG Jian1, CAO Zhouli3, DUAN Yong1, GUO Shuhai2   

  1. 1. College of Environment and Resources, Jilin University, Changchun 130012, China; 2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Shenyang 110016, China; 3. Changchun Science and Technology Innovation Center, Changchun 130012, China
  • Received:2006-06-16 Revised:1900-01-01 Online:2007-01-26 Published:2007-01-26
  • Contact: LI Yu

摘要: 添加稠油对土壤中土著微生物进行驯化, 分离出33株能以稠油为惟一碳源生长的细菌, 从中筛选出2株高效表面活性剂产生菌XJ1和SJ4, 9株高效稠油降解菌. XJ1和SJ4可将发酵液的表面张力由72.4 mN/m分别降到36.1 mN/m和36.2 mN/m;14 d摇瓶油降解率分别为35.89%和31.59%, 降解效率在各单菌中最高. 同时研究了发酵液中XJ1和SJ4的生长量与其生物表面活性剂产生情况之间的关系, 经红外光谱分析初步确定两种生物表面活性剂均为糖脂类化合物.

关键词: 生物表面活性剂, 稠油降解菌, 土壤微生物修复, 稠油

Abstract: Indigenous microbial strains of the soil were habituated by adding thick oil intentionally. Thirtythree bacterial strains were isolated with thick oil as the carbon source alone. Two higher biosurfactant producedstrains and nine higher thick oildegrading strains were screened from all the isolates. The biosurfactants produced by strain XJ1 and SJ4 in the fermentation broth could reduce the surface tension of water from 72.4 mN/mto 36.1 mN/mand 36.2 mN/m respectively. In the process of inoculating the two strains, the rates of thick oildegradation were 35.89% and 31.59% after treatment for 14 days. For strains XJ1 and SJ4, the relation between biology quantity and the surface tension of fermentation broth was studied simultaneously. Infraredspectral analytic results show the biosurfactants are both kinds of glycolipid.

Key words: biosurfactant, thick oildegrading bacteria, bioremediation of contaminated soil, thick oil

中图分类号: 

  • X132