一株多氯联苯降解菌的分离鉴定及基因分型

• 环境科学 • 上一篇

一株多氯联苯降解菌的分离鉴定及基因分型

孙立波¹,²，张兰英¹，赵喆¹，任何军¹，李爽¹，周佳欣¹

１．　吉林大学环境与资源学院，长春 130061；　２．　通辽职业学院, 内蒙古自治区通辽 028045

收稿日期:2006-10-31 修回日期:1900-01-01 出版日期:2007-07-26 发布日期:2007-07-26
通讯作者: 张兰英

Identification and Phylogenetic Analyses of a PCBs Degrading Strain

SUN Libo¹,², ZHANG Lanying¹, ZHAO Zhe¹, REN Hejun¹, LI Shuang¹, ZHOU Jiaxin¹

1. College of Environment and Resources, Jilin University, Changchun 130061, China; 2. Career College in Tongliao， Tongliao 028045， Inner Mongolia Autonomous Region,　China

Received:2006-10-31 Revised:1900-01-01 Online:2007-07-26 Published:2007-07-26
Contact: ZHANG Lanying

摘要/Abstract

摘要： 从深海底泥中分离筛选出一株以多氯连苯（PCBs）为惟一碳源和能源生长的菌株，命名为pdi. 该菌在含有PCBs 7.5 mg/L的150 mL液体培养基中培养7d，用GCMS检测其对PCBs的降解率达95.38%. 以细菌16SrDNA通用引物对pdi基因组进行PCR扩增，得到~1　500　bp的片段. 经纯化，测序后在Genbank上进行同源性比较分析及系统发育树构建，该菌与Pseudomonsa spTS1138同源性达100%，初步鉴定该菌为假单胞菌属. 对pdi基因组进一步运用RAPD分子标记技术进行随机引物扩增基因分型，获得了稳定的DNA 指纹图谱.

关键词: 多氯连苯, 16SrDNA, 生物降解, 系统发育, RAPD

Abstract: A strain pdi which can use biphenyl as the sole carbon source was isolated from seabed mud and cultured for 7 dwith 150 mL of fluid medium which contained 7.5 mg/L polychlorinated biphenyls (PCBs).　GCMS measurement showed PCBs degradation efficiency was 95.38%. The genome was amplified with bacterium 16SrDNA primer. The amplified product (about 1 500 bp) was purified and sequenced for phylogenetic analyses with Genbank. The bacterium pdi was identified as Pseudomonsa spTS1138. RAPD analyses of pdi produced stable DNA fingerprint. These studies have provided parameter for the further dynamic resolution of bacterium clusters for the rehabilitation of PCBs contaminated underground water environment with pdi PRB method.

Key words: PCBs, 16SrDNA, biodegradation, phylogenetic, RAPD

中图分类号:

X172

孙立波,，张兰英，赵喆，任何军，李爽，周佳欣. 一株多氯联苯降解菌的分离鉴定及基因分型[J]. J4, 2007, 45(04): 691-695.

SUN Libo,, ZHANG Lanying, ZHAO Zhe, REN Hejun, LI Shuang, ZHOU Jiaxin. Identification and Phylogenetic Analyses of a PCBs Degrading Strain[J]. J4, 2007, 45(04): 691-695.

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