关键词: 动态微波辅助萃取, 在线检测, 高效液相色谱, 金银花, 绿原酸

Abstract: A fast method based on dynamic microwave-assisted extraction (DMAE) coupled on-line with highperformance liquid chromatography (HPLC) by means of a flow injection interface has been developed for the determination of chlorogenic acid in Flos Lonicerae japonicae. The extraction was performed in a recirculating system. When a number of extraction cycles were completed, the fractional extract (20 μL) was driven to the analytical column by mobile phase and was measured by a UV detector. The extraction conditions were examinedand optimized. The detection and quantification limits of chlorogenic acid obtained at optimized conditionsare 0.25 and 0.83 μg/mL, respectively. The intraday and inter day precisions (RSD) obtainedare 34% and 41%, respectively. The mean recovery of chlorogenic acid is 98.1%. Compared with ultrasonic extraction used in the Chinese pharmacopoeia, the proposed method reduces the amount of solvent, provides more rapid measurement and high extraction yield.

Key words: dynamic microwaveassisted extraction, online dete ction, highperformance liquid chromatography, Flos Loniceraejaponicae, chlorogenic acid