一株产新型淀粉酶嗜温菌ZW2531-1的分离与鉴定

一株产新型淀粉酶嗜温菌ZW2531-1的分离与鉴定

王阳¹, 李樊¹, 高朝辉¹,², 郑晓一¹, 张应玖¹

1. 吉林大学分子酶学工程教育部重点实验室, 长春 130021； 2. 吉林大学生命科学学院, 长春 130012

收稿日期:2008-01-04 修回日期:1900-01-01 出版日期:2008-07-26 发布日期:2008-07-26
通讯作者: 张应玖

Isolation and Identification of a Mesophilic Bacteria ZW2531-1Producing a Novel Amylase

WANG Yang¹, LI Fan¹, GAO Chaohui¹,², ZHENG Xiaoyi¹, ZHANG Yingjiu¹

1. Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, China; 2. College of Life Science, Jilin University, Changchun 130012, China

Received:2008-01-04 Revised:1900-01-01 Online:2008-07-26 Published:2008-07-26
Contact: ZHANG Yingjiu

摘要/Abstract

摘要： 根据淀粉筛选平板上透明圈的大小, 从土壤中筛选、分离出12株产淀粉酶嗜温菌株, 由各菌株分泌淀粉酶催化淀粉降解的产物组分特征判定, 菌株ZW2531-1为初筛12个菌株中较优良的产酶菌株. 由ZW2531-1产生的新型淀粉酶可催化淀粉降解产生麦芽糖、麦芽三糖和异麦芽三糖及异麦芽四糖, 但不产生葡萄糖. 通过电镜观察, 可见ZW2531-1菌株的杆菌形态及芽孢. 提取ZW2531-1的全基因组, 根据已知16S rDNA上的保守序列设计引物, 扩增出ZW25311菌株的16S rRNA基因, 并与其他标准菌16S rDNA序列进行对比分析, 建立进化树, 最终确定了ZW25311的系统发育地位, 推断ZW2531-1应属于Bacillus属的一个种, 为Bacillus sp.ZW2531-1.

关键词: 淀粉酶, 嗜温菌, 进化树, 异麦芽低聚糖

Abstract: Twelve amylaseproducing mesophilic strains, newly isolated from Chinese soil were screened according to their ability of starch digestion by clear zone method. Among them, strain ZW2531-1 showed high amylaseproducing ability, and its amylase could catalyze the degradation of starch to produce several oligosccharides including maltose, maltotriose and a large amount of isomaltotriose and isomaltotetraose, without glucose. Strain ZW2531-1 was bacilliform, a spore forming bacterium under an electron microscope. To explorethe phylogenetic position of strain ZW2531-1, its 16S rRNA gene was amplified, sequenced, and then was compared with those of standard bacteria available in Genebank databases. Further, the phylogenetic tree including strain ZW2531-1 was built. All the results regarding strain ZW25311 support that bacterium ZW2531-1 belongs to genus Bacillus, therefore, it could be proposed as Bacillus sp. ZW2531-1.

Key words: amylase, mesophilic bacteria, phylogenetic tree, isomaltooligosaccharide

中图分类号:

Q93.331

王阳, 李樊, 高朝辉,, 郑晓一, 张应玖. 一株产新型淀粉酶嗜温菌ZW2531-1的分离与鉴定[J]. J4, 2008, 46(04): 779-783.

WANG Yang, LI Fan, GAO Chaohui,, ZHENG Xiaoyi, ZHANG Yingjiu. Isolation and Identification of a Mesophilic Bacteria ZW2531-1Producing a Novel Amylase[J]. J4, 2008, 46(04): 779-783.

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