表层沉积物中重金属形态与酶活性的相关性

表层沉积物中重金属形态与酶活性的相关性

李鱼¹,², 郭霖¹, 黎娜², 陈界江²

1. 华北电力大学能源与环境研究中心, 北京 102206； 2. 吉林大学环境与资源学院, 长春 130012

收稿日期:2008-10-14 修回日期:1900-01-01 出版日期:2009-05-26 发布日期:2009-06-23
通讯作者: 李鱼

Correlations between Fractions of Heavy Metals andEnzyme Activities in Surficical Sediments

LI Yu¹,², GUO Lin¹, LI Na², CHEN Jiejiang²

1. Energy and Environmental Research Center, North China Electric Power University, Beijing 102206, China;2. College of Environment and Resources, Jilin University, Changchun 130012, China

Received:2008-10-14 Revised:1900-01-01 Online:2009-05-26 Published:2009-06-23
Contact: LI Yu

摘要/Abstract

摘要： 利用改进的Tessier连续萃取法研究实验室模拟的表层沉积物中重金属（Fe,Mn,Cu,Zn和Pb）在一定时间（45 d）内的形态分布特征, 同时考察了沉积物体系中pH值、氧化还原电位(Eh)及其中酶（蛋白酶、脱氢酶、磷酸酶、蔗糖酶）活性的变化规律. 结果表明, 在考察的时间范围内, 沉积物体系中Mn的形态主要由可交换态向碳酸盐结合态、铁/锰氧化态转化（分别为10.2%和32.1%）, 可交换态的Zn向铁/锰氧化态转化了11.5%, 而Pb的有机质结合态则增加了20.1%； pH值初期波动较大而后趋于平缓, Eh则呈现波动状态；蛋白酶、脱氢酶的活性分别增加了88.9%和71.4%. 沉积物体系中pH值与碳酸盐结合态的Fe,Cu呈显著正相关, 蛋白酶活性与Mn的碳酸盐结合态、铁/锰氧化态, Zn的可交换态、铁/锰氧化态以及Pb的有机质结合态之间显著相关（n=8, p=0.05）, 脱氢酶活性与Pb的碳酸盐结合态也存在显著相关性（n=8, p=0.05）, 表明除pH值外, 酶活性是沉积物中重金属形态转化的一个重要影响因素.

关键词: 表层沉积物, 重金属形态, 连续萃取, 蛋白酶, 脱氢酶

Abstract: Distribution of heavy metals (Fe,Mn,Cu,Zn and Pb) surficial sediments with water phase in the simulated system in a period of 45 d w as investigated via a modified sequential extraction procedure, and pH, Eh and enzymes (protease, dyhydrogenas, sucrase and phosphatase) were monitored simultaneously. The resultsindicate that exchangeable manganese was converted into carbonateassociated fractions (10.2%) and Fe/Mnoxides fraction (32.1%), respectively, 11.5% zinc was found to exist from exchangeable to Fe/Mnoxides fraction, and organic matterassociated Pb was observed to increase by 20.1%. pH was variable in the first 20 d while Eh was fluctuated during the whole experiment. The activities of protease and dyhydrogenase were raised by a factor of 88.9% and that of 71.4%, respectively. The correlation analysis shows that pH was signifi cantly positively correlated with carbonateassociated Fe,Cu. Noticeable correlation was found between protease activity and Mn of carbonateassociated Fe/Mnoxide fraction, Zn of exchangeable Fe/Mnoxide fraction as well as Pb of organic mattersassociated fraction (n=8, p=0.05), and the similarcorrelative phenomenon was obtained between dyhydrogenase and Pb of carbonateassociated fraction, implying that the enzyme is one of the most important factors in transforma tion and cycling of heavy metals in surficial sediments besides pH.

Key words: surficial sediment, fractions of heavy metal, sequential extraction, protease, dyhydrogenase

中图分类号:

X132

李鱼,, 郭霖, 黎娜, 陈界江. 表层沉积物中重金属形态与酶活性的相关性[J]. J4, 2009, 47(03): 628-634.

LI Yu,, GUO Lin, LI Na, CHEN Jiejiang. Correlations between Fractions of Heavy Metals andEnzyme Activities in Surficical Sediments[J]. J4, 2009, 47(03): 628-634.

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