吉林大学学报(理学版)

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不同菌株培养顺序对添加黏土矿物白浆土腐殖质组成的影响

王帅1, 徐俊平1, 王楠1, 窦森2, 刘兰1, 范嘉妍1, 李芳慧1   

  1. 1. 吉林农业科技学院 农学院, 吉林 吉林 132101; 2. 吉林农业大学 资源与环境学院, 长春 130118
  • 收稿日期:2017-07-22 出版日期:2018-03-26 发布日期:2018-03-27
  • 通讯作者: 窦森 E-mail:dousen1959@126.com

Effects of Different Microbial Culture Sequences on HumicComposition of Albic Soils Amended with Clay Minerals

WANG Shuai1, XU Junping1, WANG Nan1, DOU Sen2, LIU Lan1, FAN Jiayan1, LI Fanghui1   

  1. 1. College of Agricultural Sciences, Jilin Agricultural Science and Technology University, Jilin 132101, Jilin Province,China; 2. College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, China
  • Received:2017-07-22 Online:2018-03-26 Published:2018-03-27
  • Contact: DOU Sen E-mail:dousen1959@126.com

摘要: 采用室内培养法, 以混有木质素白浆土为供试对象, 通过设置巨大芽孢杆菌(Bacillus megaterium)、 黑曲霉(Aspergillus niger)和灰色链霉菌(Streptomyces griseus)的6种培养顺序, 研究高岭石(K矿物)和蒙脱石(M矿物)对白浆土腐殖质组成的影响. 结果表明: 当以A.niger为Ⅰ期培养菌株, 或以B.megaterium为Ⅰ期、 A.niger为Ⅱ期培养菌株时, K矿物对白浆土-C(HA)-C(FA)的促进作用大于M矿物, M矿物更利于HA分子结构的复杂化, 当以S.griseus为Ⅰ期培养菌株时, M矿物更利于增加白浆土的-C(HA)C(FA); 在B.megaterium→S.griseus→A.niger培养顺序下, 与SiO2相比, 两类矿物均有利于C(HA)C(FA)的增加; 除S.griseus→B.megaterium→A.niger外, 在其他5种培养顺序下, K矿物更利于C(Hu)的形成; 当Ⅱ期培养菌株为A.niger时, M矿物比K矿物更利于白浆土总有机碳(TOC)的矿化; S.griseus→A.niger→B.megaterium培养顺序对TOC的矿化程度最弱, 且K矿物更易促进白浆土C(WSS)的消耗, 两类矿物在A.niger→B.megaterium→S.griseus培养顺序下未对C(WSS)产生差异影响, 在其他4种培养顺序下, M矿物更易促进白浆土C(WSS)的消耗; 与SiO2相比, 两类矿物均可促进白浆土C(HE)的消耗.

关键词: 菌株培养顺序, 白浆土, 木质素, 黏土矿物, 腐殖质组成

Abstract: By the indoor culture method, the albic soils amended with lignin as the tested object, we studied the effects of kaolinite or montmorillonite on the humic composition of albic soils by setting six microbial culture sequences of Bacillus subtilis, Aspergillus niger and Streptomyces griseus. The results show that the promotion effect of C(HA)C(FA) of albic soils caused by kaolinite is greater than montmorillonite when A.niger is served as the Ⅰ period cultured strain or B.megaterium is served as the Ⅰ period cultured strain and A.niger is served as the Ⅱ period cultured strain. The montmorillonite is more beneficial to making the HA molecular structure complicated, and the montmorillonite is more beneficial to the increase of the 
C(HA)C(FA) when S.griseus is served as the Ⅰ period cultured strain; under the influence of B.megaterium→S.griseus→A.niger, compared with SiO2, two kinds of minerals are beneficial to the increase of the C(HA)C(FA); in addition to S.griseus→B.megaterium→A.niger, the kaolinite is more beneficial to the formation of C(Hu) in the other five microbial culture sequences; the montmorillonite is more beneficial to the mineralization of total organic carbon (TOC) of albic soils than kaolinite when the A.niger is served as the Ⅱ period cultured strain; the degree of mineralization of TOC is the weakest in the microbial culture sequence of S.griseus→A.niger→B.megaterium, and the kaolinite is more likely to promote the microbial consumption of C(WSS); the two kinds of minerals do not affect the wC(WSS) in the ulture sequence of \%A.niger→B.megaterium→S.griseus\%, and montmorillonite is more likely to promote the microbial consumption of C(WSS) in other four kinds of culture sequence; compared with SiO2, the two kinds of minerals can promote the consumption of C(HE) of albic soils.

Key words: albic soils, lignin, microbial culture sequence, humic composition, clay mineral

中图分类号: 

  • X132