关键词: 人乳头瘤病毒16型, 人乳头瘤病毒52型, 等温多自配引发扩增, 双荧光

Abstract: The isothermal multipleselfmatchinginitiated amplification (IMSA) technology was used to detect human papillomavirus (HPV) and six specific primers were designed for HPV16E7 or HPV52E6 gene sequences. Meanwhile, dual fluorescence indicator (hydroxy naphthol blue (HNB) and SYBR Green Ⅰ) was added to the detection system. We established dual fluorescence IMSA method for rapid detection of human papillomavirus. The results show that the dual fluorescence indicator constructed by 340 μmol/L HNB and 1∶10 000 SYBR Green Ⅰ has obvious indication effect in the IMSA reaction system. The coloration of positive reaction tube is yellowgreen under the 455 nm blue excitation,  the coloration of negative reaction tube is orangered, and the detection limits of HPV16 and HPV52 are 60,600 copies/μL, respectively, HPV16 and HPV52 can be specifically detected in the samples, and there is no difference in comparison with clinical detection results.

Key words: dual fluorescence, human papillomavirus 52 (HPV52), human papillomavirus 16 (HPV16), isothermal multipleselfmatchinginitiated amplification (IMSA)