林蛙残体多肽螯合硒的制备及其抗炎活性

吉林大学学报(理学版) ›› 2025, Vol. 63 ›› Issue (3): 954-0962.

林蛙残体多肽螯合硒的制备及其抗炎活性

徐萍¹，王君淑²，鞠凤霞³，李深¹，张凤清¹

1. 长春工业大学化学与生命科学学院，长春 130012； 2. 敦化市市场监督管理局，吉林敦化 133700； 3. 吉林工商学院粮食学院，长春 130102

收稿日期:2024-05-21 出版日期:2025-05-26 发布日期:2025-05-26
通讯作者: 张凤清 E-mail:16643690575@163.com

Preparation and Anti-inflammatory Activity of Selenium Chelated by Rana dybowskii Residues Peptides

XU Ping¹, WANG Junshu², JU Fengxia³, LI Shen¹, ZHANG Fengqing¹

1. College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China; 2. Dunhua Market Supervision and Administration Bureau, Dunhua 133700, Jilin Province， China; 3. School of Grain Science and Technology， Jilin University of Commerce and Industry, Changchun 130102, China

Received:2024-05-21 Online:2025-05-26 Published:2025-05-26

摘要/Abstract

摘要： 以林蛙（Rana dybowskii）残体为原材料，在酶解条件下获得林蛙残体多肽(RDRP)，再与硒离子（Se⁴⁺）螯合制备林蛙残体多肽螯合硒(RDRPCS)，对合成的化合物进行表征，研究螯合反应的优化条件，并通过体外细胞模型对其抗炎活性进行研究. 实验结果表明： Se⁴⁺与RDRP作用生成螯合物；RDRPCS有足够的金属结合位点，螯合位点分别为氨基、羟基和C=O；螯合工艺优化结果为温度60 ℃，pH=6, 时间65 min, 质量比2.5∶1，该条件下螯合反应的效率为70.7%；不同浓度的螯合物对RAW264.7炎症细胞模型的NO，TNF-α，IL-6和IL-1β的释放量均具有抑制效果.

关键词: 林蛙残体肽, 螯合硒, 抗炎, 细胞模型

Abstract: Rana dybowskii residue was used as raw material， the Rana dybowskii residue peptides (RDRP) was abtained under enzymatic hydrolysis conditions, and then were chelated with selenium ions (Se⁴⁺) to prepare Rana dybowskii residue peptide chelated selenium (RDRPCS). We characterized the synthesized compounds, studied the optimized conditions for chelation reaction, and investigated their anti-inflammatory activity through in vitro cell models. The experimental results show that Se⁴⁺ reacts with RDRP to form chelates. RDRPCS has sufficient metal binding sites, with chelating sites being amino, hydroxyl, and C=O. The optimization result of chelation process is that temperature is 60 ℃, pH=6, time is 65 min, and a mass ratio is 2.5∶1， the efficiency of chelation reaction under this conditions is 70.7%. Different concentrations of chelates have inhibitory effects on the release of NO, TNF-α,IL-6 and IL-1β in RAW264.7 inflammatory cell model.

Key words: Rana dybowskii residues peptides, , chelating selenium, , anti-inflammatory, cell model

中图分类号:

徐萍, 王君淑, 鞠凤霞, 李深, 张凤清. 林蛙残体多肽螯合硒的制备及其抗炎活性[J]. 吉林大学学报(理学版), 2025, 63(3): 954-0962.

XU Ping, WANG Junshu, JU Fengxia, LI Shen, ZHANG Fengqing. Preparation and Anti-inflammatory Activity of Selenium Chelated by Rana dybowskii Residues Peptides[J]. Journal of Jilin University Science Edition, 2025, 63(3): 954-0962.

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