纳米氧化铝模板促进细胞色素c的电催化

纳米氧化铝模板促进细胞色素c的电催化

秦玉华，王胜天，许宏鼎

吉林大学化学学院，长春 130023

收稿日期:2003-09-15 修回日期:1900-01-01 出版日期:2004-01-26 发布日期:2004-01-26
通讯作者: 许宏鼎

Electrocatalysis of cytochrome c promoted by the nano-alumina templates

QIN Yu-hua, WANG Sheng-tian, XU Hong-ding

College of Chemistry, Jilin University, Changchun 130023, China

Received:2003-09-15 Revised:1900-01-01 Online:2004-01-26 Published:2004-01-26
Contact: XU Hong-ding

摘要/Abstract

摘要： 在草酸溶液中，通过阳极氧化铝箔制备纳米氧化铝(AAO)模板, 将细胞色素c（Cyt c）固定在纳米AAO模板和4,4-二硫二吡啶(PySSPy)修饰金电极表面，制得Cytc/Au/AAO/PySSPy薄膜电极. 在pH 6.8的缓冲溶液中, 该电极在0.059 V (vs. Ag/AgCl) 处有一对准可逆氧化还原峰, 为Cyt c血红素辅基Fe(Ⅲ)/Fe（Ⅱ）电对的特征峰. 在AAO/PySSPy薄膜的微环境中, Cyt c与金电极之间的电子传递加快. 紫外光谱结果表明， Cyt c在AAO薄膜中依然保持其原始构象. 该Cyt c/Au/AAO/PySSPy薄膜电极还可用于过氧化氢的催化还原.

关键词: 纳米氧化铝, 细胞色素c, 直接电化学, 电催化

Abstract: Cyt c/Au/AAO/PySSPy electrodes were made by immobilizing Cyt c on PySSPy modified gold electrodes covered with AAO templates. The cyclic voltammgram of Cyt c/Au/AAO/PySSPy films shows a pair of well-defined and nearly reversible peaks at about 0.059 V(vs. Ag/AgCl) in pH 6.8 PBS buffers, being the chara cteristic peaks of the Cyt c heme Fe(Ⅲ)/Fe（Ⅱ）redox couples. In the microenvironm ent of the AAO/PySSPy film, the electron transfer between Cyt c and the electrode was greatly enhanced. The soret absorption band positions suggest that Cyt c retained its native conformation in AAO templates. The hydrogen peroxide was cat alytically reduced at Cyt c/Au/AAO/PySSPy electrodes.

Key words: nano-alumina, cytochrome c, direct electrochemistry, electrochemical catalysis

中图分类号:

O657

秦玉华，王胜天，许宏鼎. 纳米氧化铝模板促进细胞色素c的电催化[J]. J4, 2004, 42(01): 113-115.

QIN Yu-hua, WANG Sheng-tian, XU Hong-ding. Electrocatalysis of cytochrome c promoted by the nano-alumina templates[J]. J4, 2004, 42(01): 113-115.

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