荧光引物扩增AMG基因鉴定古代人骨遗骸的性别

荧光引物扩增AMG基因鉴定古代人骨遗骸的性别

刘树柏¹, 崔银秋¹,2, 谢承志¹, 朱泓², 周慧^1,2

1. 吉林大学生命科学学院, 长春 130023; 2. 吉林大学边疆考古中心考古DNA实验室, 长春 130023

收稿日期:2003-09-03 修回日期:1900-01-01 出版日期:2004-01-26 发布日期:2004-01-26
通讯作者: 周慧

Sex identification of archaeological human remains based on amplification of AMG gene via fluorescent-labeled primers

LIU Shu-bai¹, CUI Yin-qiu^1,2, XIE Cheng-zhi¹, ZHU Hong², ZHOU Hui^1,2

1. College of Life Science, Jilin University, Changchun 130023, China; 2. Center of Border Archaeology, Ancient DNA Laboratory, Jilin University, Changchun 130023, China

Received:2003-09-03 Revised:1900-01-01 Online:2004-01-26 Published:2004-01-26
Contact: ZHOU Hui

摘要/Abstract

摘要： 建立一种用荧光标记引物扩增AMG基因的性染色体同源片段方法, 对古代人骨遗骸进行性别鉴定. 用其测定5个古代遗骸的性别, 其结果与形态学结果一致.

关键词: 性别鉴定, 聚合酶链式反应, AMG基因, 荧光标记引物

Abstract: A sensitive and reliable method, based on the amplification of AMG gene by fluorescent-labeled primers, was established to identify the sex of arch aeological human remains. Using this method, the sex was determined for five s ke letal remains, and the results are consistent with those of morpholo gical judgment. This sensitive and reliable method has a high value in forensic medicine, anthro pology, and archaeology.

Key words: sex identification, polymerase chain reaction（PCR）, amelogenin gene, fluorescent-labeled primers

中图分类号:

Q987

刘树柏, 崔银秋,2, 谢承志, 朱泓, 周慧. 荧光引物扩增AMG基因鉴定古代人骨遗骸的性别[J]. J4, 2004, 42(01): 139-142.

LIU Shu-bai, CUI Yin-qiu, XIE Cheng-zhi, ZHU Hong, ZHOU Hui. Sex identification of archaeological human remains based on amplification of AMG gene via fluorescent-labeled primers[J]. J4, 2004, 42(01): 139-142.

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