固相萃取GC-MS法测定蜂蜜中挥发性成分

吉林大学学报(理学版)

固相萃取GC-MS法测定蜂蜜中挥发性成分

梁芳慧^1,2, 靳丹虹², 黄新功^3, 于爱民³, 宋大千^3, 魏士刚³

1. 吉林大学吉林省光谱分析仪器工程技术研究中心, 长春 130021；2. 长春医学高等专科学校药学系, 长春 130031； 3. 吉林大学化学学院, 长春 130012

收稿日期:2014-05-04 出版日期:2015-01-26 发布日期:2015-01-19
通讯作者: 魏士刚 E-mail:weisg@jlu.edu.cn

Volatile Compounds from Honey Analyzed bySolid Phase Extraction and GCMS

LIANG Fanghui^1,2, JIN Danhong^2, HUANG Xingong^3, YU Aimin^3, SONG Daqian^3, WEI Shigang³

1. Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University,Changchun 130021, China； 2. Department of Pharmacy, Changchun Medical College, Changchun 130031, China；3. College of Chemistry, Jilin University, Changchun 130012, China

Received:2014-05-04 Online:2015-01-26 Published:2015-01-19
Contact: WEI Shigang E-mail:weisg@jlu.edu.cn

摘要/Abstract

摘要：

通过比较溶剂萃取(SE)、同时蒸馏萃取(SDE)和固相萃取(SPE)3种样品前处理方法, 确定蜂蜜中挥发性成分的最优提取方法, 建立蜂蜜中挥发性成分的固相萃取气相色谱质谱(GCMS)检测方法. 以亲水性HLB固相萃取柱为对象, 考察并优化HLB柱的淋洗与洗脱条件, 确定HLB柱在以1 mL的V(甲醇)∶V(水)=1为淋洗剂, 1 mL二氯甲烷为洗脱剂的条件下对蜂蜜中挥发性成分提取效果最好, GCMS可分析出蜂蜜中47种挥发性成分.

关键词: 蜂蜜, 挥发性成分, 固相萃取, 气相色谱质谱联用

Abstract:

Based on GCMS coupled with each of three pretreatment methods solvent extraction (SE), simultaneous steam distillationextraction (SDE) and solid phase extraction (SPE), a novel approach for the determination of volatile organic compounds (VOCs) in honey was established. Especially, we focused on the SPE from HLB minicolumn. The effects of various solvents for wash and elution were investigated, and the conditions of the HLB SPE were optimized. The results showed that 47 kinds of VOCs could be identified with the washing by V(methanol)∶V(water)=1 followed by the elution by dichloromethane.

Key words: honey, volatile organic compounds, solid phase extraction(SPE), gas chromatographymass spectrometry(GCMS)

中图分类号:

O657.63

梁芳慧, 靳丹虹, 黄新功, 于爱民, 宋大千, 魏士刚. 固相萃取GC-MS法测定蜂蜜中挥发性成分[J]. 吉林大学学报(理学版), 2015, 53(01): 142-147.

LIANG Fanghui, JIN Danhong, HUANG Xingong, YU Aimin, SONG Daqian, WEI Shigang. Volatile Compounds from Honey Analyzed bySolid Phase Extraction and GCMS[J]. Journal of Jilin University Science Edition, 2015, 53(01): 142-147.