秀丽线虫sod-3基因RNA干扰及早衰模型的建立

秀丽线虫sod-3基因RNA干扰及早衰模型的建立

关树文¹，黄磊¹，罗晶¹，韩秋菊²，王丽萍¹，李惟¹

1. 吉林大学生命科学学院，长春 130021； 2. 东北师范大学遗传与细胞研究所，长春 130021

收稿日期:2006-09-19 修回日期:1900-01-01 出版日期:2007-07-26 发布日期:2007-07-26
通讯作者: 王丽萍

Model of Rapid Aging Based on RNAiMediated Gene Silencing of sod3 Expression in Caenorhabditis Elegans

GUAN Shuwen¹, HUANG Lei¹, LUO Jing¹, HAN Qiuju², WANG Liping¹, LI Wei¹

1. Collge of Life Science, Jilin University, Changchun 130021, China; 2. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130021, China

Received:2006-09-19 Revised:1900-01-01 Online:2007-07-26 Published:2007-07-26
Contact: WANG Liping

摘要/Abstract

摘要： 利用RNAi技术，通过干扰秀丽线虫中的sod-3基因表达，建立了因超氧化物歧化酶3（SOD3）表达缺失引起快速衰老的线虫模型，为sod-3 基因功能的研究以及相关疾病的基因治疗等提供动物模型.

关键词: RNAi, 秀丽线虫, 超氧化物歧化酶3

Abstract: According to the oxidative damage theory of aging, free radicals, particularly reactive oxygen species (ROS), are responsible for the senescence and many human diseases. ROS are generated during normal metabolic process in all the oxygenutilizing organisms. They are detoxified by superoxide dismutase. The manganesecontaining superoxide dimutase 3 (SOD3) has been shown to play a role in the aging process in  Caenorhabditis elegans. In this research, we show that sod-3 RNA interference (RNAi) inducesinsenecencein Caenorhabditis elegansas expected. This method can be used to understand the process of aging and the mechanism of antioxidance.

Key words: RNAi, Caenorhabditis elegans,  superoxide dismutase 3

中图分类号:

Q789

关树文，黄磊，罗晶，韩秋菊，王丽萍，李惟. 秀丽线虫sod-3基因RNA干扰及早衰模型的建立[J]. J4, 2007, 45(04): 676-680.

GUAN Shuwen, HUANG Lei, LUO Jing, HAN Qiuju, WANG Liping, LI Wei. Model of Rapid Aging Based on RNAiMediated Gene Silencing of sod3 Expression in Caenorhabditis Elegans[J]. J4, 2007, 45(04): 676-680.

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