J4 ›› 2011, Vol. 49 ›› Issue (01): 153-157.

• 环境科学 • 上一篇    

长春南湖水耐冷菌的筛选及其产酶特性

黄继国, 杨柳, 王玉, 朱婧, 丛伟   

  1. 吉林大学 环境与资源学院, 长春 130012
  • 收稿日期:2010-04-29 出版日期:2011-01-26 发布日期:2011-02-19
  • 通讯作者: 黄继国 E-mail:huangjg@jlu.edu.cn

Screening and Properties of Enzyme Production ofPsychrotrophs from Nanhu Lake of Changchun

HUANG Jiguo, YANG Liu, WANG Yu, ZHU Jing, CONG Wei   

  1. College of Environment and Resources, Jilin University, Changchun 130012, China
  • Received:2010-04-29 Online:2011-01-26 Published:2011-02-19
  • Contact: HUANG Jiguo E-mail:huangjg@jlu.edu.cn

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摘要：

采用低温环境下的南湖湖水为样品, 稀释后涂布平板进行富集培养, 并进行细菌菌落形态及生理生化性质测定. 实验共培养出耐冷菌35株, 包括细菌14株, 真菌21株. 其中细菌分属于乳杆菌属、 葡萄球菌属、 糖球菌属、 放线菌属; 真菌分属于霉菌和酵母菌. 分离的菌种能产生多种大分子物质水解酶类, 包括氧化酶、 接触酶、 淀粉水解酶、 明胶酶等, 并且大多数菌种具有硝酸盐还原作用阳性, 还有一些耐冷菌具有亚硝酸盐还原作用阳性、 反硝化作用阳性等.

关键词: 湖泊； 低温菌； 鉴定, 性质

Abstract:

The samples from  the South Lake at a low temperature were taken and diluted onto coated tablet for concentration inoculation. A total of 35 coldadaptedstrains were isolated, which contained 14 bacteria and 21 fungi. The colony morphology and physiological and biochemical results show that the psychrotrophic bacteria belong to Lactobacilus, Saccharococcus, Staphyloccus, Actinomyces and the fungi belong to molds and yeast. Some of them can produce hydrolases including oxidase, catalase, amylase and gelatinase, and some of psychrotrophs can make nitrate reduction, nitrite reduction and so on.

Key words: lake, psychrotrophic bacteria, identification, character

中图分类号: 

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