黑种草籽调节黑素合成的药效物质基础

J4 ›› 2011, Vol. 49 ›› Issue (04): 787-791.

黑种草籽调节黑素合成的药效物质基础

范雪琦¹, 徐建国², 廖莎¹, 周佳¹, 杨伟峻³, 尚靖¹

1. 中国药科大学新药筛选中心, 南京 210009； 2. 新疆维吾尔自治区卫生厅, 乌鲁木齐 830001；3. 新疆维吾尔自治区药物研究所, 乌鲁木齐 830004

收稿日期:2010-12-30 出版日期:2011-07-26 发布日期:2011-08-16
通讯作者: 尚靖 E-mail:shangjing2006@yahoo.com.cn

Pharmacodyamic Material Basis ofNigella sativa L.Regulating Melanogenesis

FAN Xueqi¹, XU Jianguo², LIAO Sha^1, ZHOU Jia¹, YANG Weijun³, SHANG Jing¹

1. New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, China;2. Bureau of Health, Xinjiang Uyghur Autonomous Region, Urumqi 830001, China;3. Xingjiang Institute of Material Medical, Urumqi 830004, China

Received:2010-12-30 Online:2011-07-26 Published:2011-08-16
Contact: SHANG Jing E-mail:shangjing2006@yahoo.com.cn

摘要/Abstract

摘要：

以高效液相色谱(HPLC)法对黑种草籽不同体积分数的醇提取物进行色谱峰表征, 以体外培养的小鼠B16黑素瘤细胞为模型, 采用MTT法测定黑种草籽
不同体积分数的醇提取物对B16黑素瘤细胞增殖的影响, 采用LDOPA氧化法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞酪氨酸活性的影响, 采用NaOH裂解法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞黑色素含量的影响, 并结合药理研究结果对不同体积分数醇提取物的HPLC图谱进行相关性统计分析. 实验结果表明: 黑种草籽体积分数为50%的乙醇提取物具有促进小鼠黑素瘤细胞B16增殖及促进黑素合成的作用；谱效分析HPLC图谱中的6个色谱峰对药效的贡献度无差异, 表明黑种草籽药效是中药多组分协同作用.

关键词: 黑种草籽；细胞增殖；酪氨酸酶活；黑素合成；相关分析

Abstract:

Chromatographic peaks of different concentration of Nigella sativa L.extacts were characterized by HPLC. B16 murine melanoma cells were used as an in vitro cultured model. MTT was used to study the effect of Nigella sativa L.ethanolic extracts on the proliferation of B16 murine me
lanoma cells. Oxidation rate of LDOPA was measured to estimate the effect of Nigella sativa L.ethanolic extracts on tyrosinase activity of B16 murine melanoma cells. NaOH cleavage method was performed to study the effect of Nigella sativa L.ethanolic extracts on the melanogenesis of B16 murine melanoma cells. Statistical analysis to the chromatographic peaks of different concentration of Nigella sativa L.extacts and pharmacodynamic data for correlation analysis. Results show that 50% ethanolic extract of Nigella sativa L.enhances me lanogenesis and growing rate of B16 murine melanoma cells. Componenteffect relationship shows that there is no significant difference in the 6 chromatographic peaks on pharmacodynamic effect. Pharmacodynamic effect is due to the multiplecomonents of Nigella sativa L.

Key words: Nigella sativa L., proliferation, tyrosinase activity, melanogenesis, correlation analysis

中图分类号:

R965.1

范雪琦, 徐建国, 廖莎, 周佳, 杨伟峻, 尚靖. 黑种草籽调节黑素合成的药效物质基础[J]. J4, 2011, 49(04): 787-791.

FAN Xue-Qi, XU Jian-Guo, LIAO Sha, ZHOU Jia, YANG Wei-Jun, CHANG Jing. Pharmacodyamic Material Basis ofNigella sativa L.Regulating Melanogenesis[J]. J4, 2011, 49(04): 787-791.