DhHP-6延长秀丽线虫寿命的作用机制

J4 ›› 2012, Vol. 50 ›› Issue (05): 1045-1048.

DhHP-6延长秀丽线虫寿命的作用机制

王丽萍¹, 金鑫¹, 黄磊¹, 李鹏飞¹, 王立成²

1. 吉林大学生命科学学院, 长春 130012|2. 吉林大学化学学院, 长春 130012

收稿日期:2012-02-21 出版日期:2012-09-26 发布日期:2012-09-29
通讯作者: 王立成 E-mail:wlc@jlu.edu.cn

Mechanism of DhHP6 on Extending |Life Span in Caenorhabditis elegans

WANG Li ping¹, JIN Xin¹, HUANG Lei¹, LI Peng fei¹, WANG Li cheng²

1. College of Life Science, Jilin University, Changchun 130012, China;2. College of Chemistry, Jilin University, Changchun 130012, China

Received:2012-02-21 Online:2012-09-26 Published:2012-09-29
Contact: WANG Li cheng E-mail:wlc@jlu.edu.cn

摘要/Abstract

摘要：

考察次血红素六肽(DhHP-6)对秀丽线虫寿命的影响及其与DAF-16(abnormal dauer -formation-16)的关系. [JP2]实验结果表明: DhHP-6给药可提高野生秀丽线虫的平均寿命约20%, 未影响DAF-16表达缺失型秀丽线虫的平均寿命; DhHP-6可促进DAF-16入核, 启动下游基因表达; DhHP-6给药可增强DAF-16的转录活性, 提高sod-3表达量, 降低线虫体内的自由基含量.

关键词: 次血红素六肽； daf-16；寿命；抗衰老, 秀丽线虫

Abstract:

The purpose of the present work is to investigate the potential role of DhHP-6 with the help of DAF-16 in the lifespan regulation of Caenorhabditis elegans. Our results indicate that DhHP-6 can effectively increase the average lifespan of C.elegans by a factor of over 20%. Nevertheless the lifespan extending effect of DhHP-6 is inhibited by daf16 mutant strains, which implies the effect of DhHP-6 uponlongevity is associated with DAF-16. Our data demonstrate that DhHP6 activated the nucleic translocation of DAF-16 and enhanced the transcription of daf-16 downstream gene sod-3. Our results also show that the ROS levels of nematodes in vivo is specifically reduced under DhHP-6 treatment. These results suggest that DhHP-6 may extend the lifespan of C.elegans by reducing the ROS levels through DAF-16.

Key words: DhHP-6, daf-16, longevity, anti-aging, Caenorhabditis elegans

中图分类号:

Q559

王丽萍, 金鑫, 黄磊, 李鹏飞, 王立成. DhHP-6延长秀丽线虫寿命的作用机制[J]. J4, 2012, 50(05): 1045-1048.

WANG Li-Ping, JIN Xin, HUANG Lei, LI Feng-Fei, WANG Li-Cheng. Mechanism of DhHP6 on Extending |Life Span in Caenorhabditis elegans[J]. J4, 2012, 50(05): 1045-1048.

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