关键词: 四翅滨藜； LRR； 酵母表达； 抗逆性

Abstract:

A full length leucinerich repeat receptorlike protein kinase （LRR-RLK） was obtained from a fulllength Atriplex canescens cDNA library, named as
AcLRR (GenBank accession number: JN974247). To investigate the stress tolerance of AcLRR, the recombinant plasmid pYES-AcLRR was constructed by inserting the AcLRR gene into the yeast expression vector pYES\|DEST52 via LR recombination. The recombinant plasmid pYES-AcLRR that was transformed into yeast Saccharomyces cerevisiae INVSc1, and the INVSc1 strain transformed with empty pYESDEST52 was used as the control. The recombinant yeast INVSc1 harboring pYES-AcLRR and the control INVSc1 harboring empty pYESDEST52 were treated with salinealkali, drought, high temperature, frezzing and paraquat, respectively, and their survival rates were compared under these stress conditions. The results show that the survival rates of the recombinant yeast INVScl (pYES-AcLRR) were higher than those of the control strain under these stress conditions, indicating that the AcLRR was tolerant to NaCl,KCl,NaHCO₃,Na₂CO₃, drought, high temperature, frezzing stresses, which exhibits that AcLRR may play an important role in stress tolerance of A.canescens.

Key words: Atriplex canescens, LRR, yeast expression, stress tolerance