关键词: 紫苏梗提取物, 高效液相色谱, 抗氧化作用

Abstract: The effective components of Perilla stem were extracted by ethyl acetate, chloroformane, nbutanol and water respectively, the organic acids, triterpenoids and flavonoids were obtained, and its antioxidant effect was studied. The results show that the gallic acid is 0.6 g/kg in n-butyl alcohol of Perilla stem, and the oleanolic acid is 0.9 g/kg in chloroform of Perilla stem. There are lots of brass compounds in Perilla stem, and the hyperin are 1.2 g/kg in ethyl acetate and 1.1 g/kg in chloroform. All of the Perilla stem extracts have certain ability to remove ·OH, the super oxygen free radical (·O-2) and DPPH·. The removal ability of acetic ether and chloroform in 1 mg/mL are best, which reach 79% and 73%, 62% and 59%, 83% and 80% respectively. Acetic ether and chloroform have strong ability to remove ·OH radicals and DPPH·. Therefore Perilla stem extract has obvious antioxidant effect.

Key words: Perilla stem extract, high performance liquid chromatography (HPLC), antioxidant effect