采用S/W/O/W法制备BSA-PLG微球

J4 ›› 2009, Vol. 47 ›› Issue (05): 1086-1090.

采用S/W/O/W法制备BSA-PLG微球

夏洪男, 孙亚东, 王潇潇, 汪静, 付瑜, 苏正兴, 李又欣

吉林大学生命科学学院, 长春 130012

收稿日期:2009-03-23 出版日期:2009-09-26 发布日期:2009-11-03
通讯作者: 李又欣 E-mail:liyouxin@jlu.edu.cn.

Preparation of BSA-PLG Microspheres by S/W/O/W Method

XIA Hongnan, SUN Yadong, WANG Xiaoxiao, WANG Jing, FU Yu, SU Zhengxing, LI Youxin

College of Life Sciences, Jilin University, Changchun 130012, China

Received:2009-03-23 Online:2009-09-26 Published:2009-11-03
Contact: LI Youxin E-mail:liyouxin@jlu.edu.cn.

摘要/Abstract

摘要：

以生物可降解材料PLG607为载体, 采用S/W/O/W溶剂挥发法制备牛血清白蛋白(BSA)微球, 对其性质进行表征, 并考察了处方中不同添加剂对BSA-PLG微球粒径、包封率和体外释放的影响. 结果表明, 采用S/W/O/W法所得微球粒径为40～70 μm, 表面光滑圆整. 加入添加剂（m(羟丙基β环糊精) ∶m(牛血清白蛋白)=1 ∶1）后包封率可以从27.9%提高到46.3%, 加入添加剂羟丙基-β-环糊精和羟基磷灰石制备蛋白微球, 能减小突释, 提高蛋白的包封率, 改善体外释放行为, 达到均匀释放.

关键词: S/W/O/W法；聚乳酸羟基乙酸；牛血清白蛋白；微球

Abstract:

The BSAPLG microspheres were prepared by S/W/O/W emulsion solvent evaporation method with biodegradable material PLG607 as the carrier. Drug encapsulation capacity, morphology, particle size characteristics, as well as invitro release profiles of the microspheres were discussed. The results show that the microspheres were spherical and smooth and the mean size of the microspheres was 40~70 μm, with a mono dispersibility. The invitro release profiles of the microspheres produced from different methods were significantly distinct. With the additives of hydroxypropylβcyclodextrin (HPB) and hydroxyapatite (HAP), the encapsulation of microspheres increased from 27.9% to 46.3%. Therefore, using S/W/O/W method and adding additives HAP and HPB in the formulation could decrease the initial burst release of drugs from the microspheres and increase the encapsulation capacity of the BSAPLG microspheres, the invitro release profiles of which were fitted into near zero order release kinetics.

Key words: S/W/O/W method, poly(lactide\, co\, glicolide), bovine serum albumin, microsphere

中图分类号:

R944.9

夏洪男, 孙亚东, 王潇潇, 汪静, 付瑜, 苏正兴, 李又欣. 采用S/W/O/W法制备BSA-PLG微球[J]. J4, 2009, 47(05): 1086-1090.

JIA Hong-Nan, SUN E-Dong, WANG Xiao-Xiao, HONG Jing, FU Yu, SU Zheng-Xin, LI You-Xin. Preparation of BSA-PLG Microspheres by S/W/O/W Method[J]. J4, 2009, 47(05): 1086-1090.