关键词: Met e1蛋白质,  , B细胞表位, T细胞表位, 抗原表位筛选

Abstract: Online and offline software were used to predict,  screen and assist in the calculation of the B-cell and T-cell epitopes of the allergen Met e1 from Metapenaeus ensis. Firstly, the amino acid sequence of Met e1 protein was retrieved from the uniprot protein database. Secondly, the physicochemical properties,  signal peptides,  and transmembrane regions of Met e1 were analyzed  by using online tools ExPASY ProtParam,  SignalP-5.0 Server,  and TMHMM Server v.2.0. The secondary structure of Met e1 was jointly predicted and  calculated by using the PSIPRED online tool,  SOPMA online tool,  and DNAstar software,  while the tertiary structure of  Met e1 was predicted and calculated by using Swiss model online software. Thirdly, the linear B-cell epitopes were comprehensively predicted and calculated by using DNAStar offline software and IEDB online software,  CD4+T and CD8+T-cell epitopes were predicted and calculated by using the IEDB online software.  Finally, the obtained results were  screened for B-cell and T-cell epitopes. The results show  that the B-cell epitopes of Met e1 protein are located at amino acids 15—28， 45—49， 92—95， 125—130， 150—153 and 255—258,  and T-cell epitopes are located at amino acids 78—84 and 223—232.

Key words: Met e1 protein,  , B-cell epitope,  , T-cell epitope,  , antigen epitope screening