Abstract: We mensurated the lipase activity of Plancoccus Migula fermentation liquid , and studied the effects of all kinds of growth elements on the lipase yield. The result shows that the optimum conditions to yield lipase are that carbon source is glucose ; nitrogen source is peptone ; initial pH is 6.0 , and while K²HPO⁴, KH²PO⁴ ,CaCl² , ZnSO⁴ and NaCl can promote lipase production ; CuSO⁴ and MgSO⁴ can partly decrease lipase output ; FeCl³ , BaCl² , CoSO⁴ and MnSO⁴ inhibit lipase production completely. In addition non-ionic surface-active agents for instance Tween also promote lipase production.

Key words: plancoccus migula, lipase in vitro, enzyme activity