Abstract: Two methods of extracting Lilium brownii polysaccharide, hot-water method and combined-enzyme method, were studied and the optimum conditions of t he two methods were found. The yield of the polysaccharides extracted by combine d　enzymes was 2.8 times that of polysaccharides extracted by hot-water. It was also found that the antioxidative activity of the polysaccharides, such as i nhibiting effects on O^-₂·, ·OH, the hemolysis of human R BC induced by H₂O₂ and the formation of MDA, extract ed by combined-enzymes were much greater than that of those extracted by hot-wa ter. After extracted by the two methods, the polysaccharides were separated by s ephedex G-75 gel filtration chromotography and three fractions were eluted out. LBP-3, whose molecular weight was about 13 400 and had the highest antioxida tive activity of the three fractions, was found to exist in polysaccharides extr ated by combined　enzymes much more than that to exist in those extracted by hot-water.

Key words: Lilium brownii polysaccharide, combined-enzyme method, antioxide, LBP-3