Abstract: To get soluble form of single-chain Fv of antibody (scFv) and generate selenium-containing scFv with glutathione peroxidase (GPX) activity, scFv expression vector pTMF-2F3 was replaced by a new vector pRose-2F3. Non-essential amino acids of 18 were removed from pTMF-2F3 and a signal peptide was introduced in t he pRose at the N end of 2F3. The 2F3 antibody was expressed as a soluble prote in in the periplasm cavity of E. coli BL21 (lys S). The purified product was con firmed by Western Blotting. Se-2F3-ScFv is generated by the chemical mutatio n of 2F3-ScFv. The GPX activity of Se-2F3-ScFv is 2　530 U/μmol. For studying its protection of epidermal cell from UVB damage, the effects of UVB and Se-2F3- ScFv on lipid peroxidation, the cell viability and cell membrane integrity were investigat ed. It was found that Se-2F3-ScFv possessed strong ability to prevent epidermal cells from UVB damage and was an effective membrane peroxidation inhibitor.

Key words: glutathione peroxidase, single-chain variable fragment of antibody, soluble expression, antioxidation