原核表达载体pHsaE1αβ的构建和人丙酮酸脱氢酶的表达与纯化

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Construction of Prokaryotic Expression Vector pHsaE1αβ and Expression and Purification of Human Pyruvate Dehydrogenase

WU Yong-ge¹, HUANG Chang-yi², LI Wei¹, Ronald Dugglby²

1. Vaccine Research Center, College of Life Science, Jilin University, Changchun 130012, China; 2. Department of Biochemistry and Molecular Biology, University of Queensland, Brisbane QLD 4072, Australia

Received:2004-10-18 Revised:1900-01-01 Online:2005-07-26
Contact: WU Yong-ge

Abstract

CLC Number:

Q782

WU Yong-ge, HUANG Chang-yi, LI Wei, Ronald Dugglby. Construction of Prokaryotic Expression Vector pHsaE1αβ and Expression and Purification of Human Pyruvate Dehydrogenase[J].J4, 2005, 43(04): 533-537.

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Construction of Prokaryotic Expression Vector pHsaE1αβ and Expression and Purification of Human Pyruvate Dehydrogenase

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