ISSR 和 RAPDPCR技术对东北地区主推玉米品种的鉴别

Indentification of Varieties among Eitheen Northeast DominatMaizeby ISSR and RAPD-PCR Methods

LI Shang-yu¹, WANG Chun-yan², ZHANG Chao-yan³, LI Xiao-kun⁴,⁵

1. Changchun Department for Product Quality Supervision and Testing, Changchun 130012, China; 2. School of Public Health, Jilin University, Changchun 130021, China; 3. Department of Biology, Changchun Teacher College, Changchun 130032, China; 4. College of Bioengineeringand Technology, Jilin Agricultural University, Changchun 130118, China; 5. School of Biology and Phamaceutical, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China

Received:2005-08-09 Revised:1900-01-01 Online:2006-05-26 Published:2006-05-26
Contact: LI Xiao-kun

Abstract

Abstract: The gene maps of eighteen maize samples were analyzed by intersimple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) marker methods. The ISSR method was the primary method and was validated by the RAPD method. 4 highly polymorphic primers were screened out from 30 random ISSR primers and 2 highly polymorphic primers were screened out from 30 random RAPD primers. In conclusion, uniting ISSR combined with RAPD can raise the veracity of indentification.

Key words: maize,  ISSR, RAPD, indentification of varieties

CLC Number:

S513033

LI Shang-yu, WANG Chun-yan, ZHANG Chao-yan, LI Xiao-kun,. Indentification of Varieties among Eitheen Northeast DominatMaizeby ISSR and RAPD-PCR Methods[J].J4, 2006, 44(03): 509-512.

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Indentification of Varieties among Eitheen Northeast DominatMaizeby ISSR and RAPD-PCR Methods

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