Abstract: The amplification capability, the heat tolerance and the ability to long PCR of Tgo DNA polymerase were examined. The results reveal that Tgo DNA polym erase has the same amplification function asTaq enzyme but with a high fidelity. It also has a strong resistance to heat. Its amplification function would not be notably affected at 95 ℃ for 40 min. Via the recombinant enzyme, the 5.3　kb segment of Timeless gene was successfully amplified from Arabidopsis under the general conditions of PCR.

Key words: recombinant Tgo DNA polymerase, amplification capability, heat tolerance, P CR