Abstract:

We reported an investigation designed to knockout the MSTN gene by gene targeting in ovine myoblast cells. To increase the gene target efficiency and to supply nuleus transplantation donator for somatic cloning, two double traps (promotertrap and polyA trap) and targeting vectors MSTNgreen fluorescent protein (GFP) and MSTNneo were constructed and the foetal and neonatal ovine primary myoblast were cultured and identified. The cell cultural conditions were optimized. At the same time, the construct of no promoter vector was easy to molecular identification of positive cell clone after gene targetting.

Key words: promotertrap targeting vetor, Myostatin, myoblast cell, gene targenting