Abstract:

To investigate the antioxidant effect of Se-scFv-B3, a model system of H₂O₂induced rat cardiac myocyte damage was established. Se-scFv-B3 was demonstrated to protect cardiac myocytes against H₂O₂induced damage by the increase of cell viability, the decline of cell apoptosis, the inhibition of mitochondrial membrane potential (Δψ_m) collapse, the decrease of Caspase-3 activity and intracellular H₂O₂ after treatment of rat cardiac myocytes by Se-scFv-B3.

Key words: oxidative stress, apoptosis, Se-scFv-B3, cardiac myocytes, hydrogen peroxide