Abstract:

According to the DNA sequence of the F1L gene which was stored in GenBank, the authors designed one pair of primers to amplify F1L gene of JLSY04 by PCR. The amplified positive production was sequenced and the sequence of it was compared with the sequences of several reference strains domesticisolated. The results show that JLSY04 stain and OV/Torino strain show the lowest homology of 960% with respect to F1L gene, and the highest homology of Jilin strain was up to 994%. There was little difference of F1L gene between JLSY04 strain and each of other reference strains isolated from different species and different areas in China. The analysis result of homology suggests that the F1L gene can be used as the target genes of the genetically engineered vaccine.

Key words: Orf virus, F1L gene, cloning, homology analysis