Abstract:

To detect feline calicivirus (FCV), a realtime quantitative PCR (qPCR) assay was established by using a pair of primers and TaqMan probe designed and synthesized according to the conserved capsid protein gene ORF2 sequence of FCV in the GenBank. The reaction system and conditions of the qPCR assay were optimized and the standard curve was established. Further, the specificity, sensitivity and repeatability test were also assessed. The established quantitative PCR assay was applied to detecting clinical samples infected by FCV. The results show that the correlation rate of the standard curve for the qPCR was 0.992. The detected quantity was from 2.26×10¹ to 2.26×10¹¹ copies/μL of FCV cDNA. With the qPCR method, a reliable diagnostic result was obtained for detecting FCV samples. But detection of other feline pathogenic agents was negative. In addition, reproducible assay showed a good performance of repeatability and the variation coefficient of intragroup was 2.158%.

Key words: feline calicivirus(FCV), flurogenic quantitative polymerase chain reaction, detection method