林蛙输卵管总RNA提取方法的优化及RT-PCR验证

Abstract

Abstract:

We compared the extraction effects of improved CTAB, TRIzol and RNAiso Plus, and investigated the extraction yield with different methods. It presents the advantages of high quality total RNA extracted by the optimized method, A₂₆₀/A₂₈₀ ratio was 196 and yield was more than 561 μg/g. Through reverse transcription\|polymerase chain reaction (RT-PCR), we got GAPDH and EF-1-α- sequences.

Key words: Rana chensinensis oviduct, RNA extraction, optimization, RT-PCR, validation

CLC Number:

Q752

LI Jiahui, LIU Huimin, CHEN Wenchao, LIU Jingsheng. Optimization of Total RNA Isolation from Rana chensinensisOviduct and Validate the Method by RT-PCR[J].Journal of Jilin University Science Edition, 2014, 52(03): 605-610.

[1]	WANG Songhua, XIA Shi, LI Yong. Convergence of a Class of Modified Hager-Zhang Conjugate Gradient Method and Its Numerical Experiment [J]. Journal of Jilin University Science Edition, 2021, 59(5): 1107-1116.
[2]	FU Bo, WANG Ruizi, WANG Liyan, ZHANG Xiangyi. Enhancement Method of Underwater Color Cast Image Based on Deep Convolutional Neural Network [J]. Journal of Jilin University Science Edition, 2021, 59(4): 891-899.
[3]	ZENG Hongzhi, SHI Hongsong. Network Intrusion Detection Method Based on Combination of Semi-supervised Technology and Active Learning [J]. Journal of Jilin University Science Edition, 2021, 59(4): 936-942.
[4]	MO Xiaoqing, SUN Xiangkai. Robust Approximate Optimality for a Class of Uncertain Semi-infinite Multi-objective Optimization Problems [J]. Journal of Jilin University Science Edition, 2021, 59(2): 257-262.
[5]	HU Chuanfeng, REN Jingwen, HU Hui, LIN Hongwei. Topology Optimization for Parametric Porous Structure Based on Iso-Geometric Analysis [J]. Journal of Jilin University Science Edition, 2021, 59(1): 65-76.
[6]	ZHANG Qiang, ZHU Liutao, WANG Ying. Cultural Shuffled Frog Leaping Algorithm for Continuous Space Optimization Problem [J]. Journal of Jilin University Science Edition, 2020, 58(6): 1443-1451.
[7]	CHEN Jing, TANG Aotian, LIU Zhen, XU Sen, CAO Xiaocong. A Multi-objective Particle Swarm Optimization Algorithm Based on Kriging Model Infilling Strategy [J]. Journal of Jilin University Science Edition, 2020, 58(5): 1159-1166.
[8]	YU Shengbao, FANG Yu, GAO Lihui, DIAO Shu. SHEPWM Control Method of Controllable Emission Current Frequency Based on Improved Particle Swarm Optimization Algorithm [J]. Journal of Jilin University Science Edition, 2020, 58(5): 1207-1214.
[9]	TANG Zhanyong, HAO Jie, GUO Jun, LIU Baoying. Dynamic Analysis of IC Power Network Based onVariable Step Size Random Walk Algorithm [J]. Journal of Jilin University Science Edition, 2020, 58(4): 868-876.
[10]	YUAN Kaiyin, WANG Feng. Security Risk Assessment of Wireless Sensor NetworksBased on Index Selection and Weighted Fusion [J]. Journal of Jilin University Science Edition, 2020, 58(4): 937-943.
[11]	DIAO Xinliu, LIU Hongwei, ZHAO Ting. An Improved ThreeDimensional SubspaceMinimization Conjugate Gradient Method [J]. Journal of Jilin University Science Edition, 2020, 58(3): 470-478.
[12]	JIN Xiaomin, ZHANG Liping, LI Huijing. Fast Image Segmentation Method for Controlling Evolution of Active Contour [J]. Journal of Jilin University Science Edition, 2020, 58(3): 639-644.
[13]	WANG Bo, YU Zhezhou, YUAN Jun, FU Hong, YU Jianqun. New Type of CAE Software Based on MBD and DEM Coupling [J]. Journal of Jilin University Science Edition, 2020, 58(2): 371-378.
[14]	SHI Xiaobing, GUO Degui, CAO Jie. Radio Transmitter Location Method Based onAdaptive Hata Model [J]. Journal of Jilin University Science Edition, 2020, 58(1): 109-112.
[15]	GUO Yujie, ZHANG Qiang, YUAN Heping. DualPopulation Cooperative Multiobjective Particle SwarmOptimization Algorithm and Its Application#br# #br# [J]. Journal of Jilin University Science Edition, 2019, 57(5): 1155-1162.

Optimization of Total RNA Isolation from Rana chensinensisOviduct and Validate the Method by RT-PCR

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10