2-TeCD对UVB致NIH3T3细胞损伤的保护作用

Journal of Jilin University Science Edition

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Protective Effect of 2-TeCD on UVBInduced Damage in NIH3T3 Cells

YIN Juxin¹, GONG Pingsheng¹, TONG Yi², WU Yandong², WU Lida², LV Shaowu^1,3, YAN Ganglin^1,3

1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University,Changchun 130012, China; 2. Corn Deep Processing of National Engineering Research Center,Jilin COFCO Biochemical Co., Ltd., Changchun 130033, China;3. College of Life Sciences, Jilin University, Changchun 130012, China

Received:2016-03-08 Online:2016-11-26 Published:2016-11-29
Contact: LV Shaowu E-mail:lvsw@jlu.edu.cn

Abstract

Abstract: Using fluorescence staining of 4,6diamidino2phenylindole dihyodrochloride and flow cytometry we studied protective effect of an artificial mimic 2tellurium bridged cyclodextrin (2-TeCD) with the activity of glutathione peroxidase (GPx) on UVBinduced damage in NIH3T3 cells. The results show that 2TeCD can affect DNA lysis rate, the level of lipid peroxidation, the volume fraction of ROS, cell cycle, P53 protein expression and other indicators in NIH3T3 cells caused by UVB. The 2TeCD can play a protective role against UVB damage by removing free radicals.

Key words: UVB, gluathione peroxidase (GPx), mimic, 2-TeCD

CLC Number:

Q559

YIN Juxin, GONG Pingsheng, TONG Yi, WU Yandong, WU Lida, LV Shaowu, YAN Ganglin. Protective Effect of 2-TeCD on UVBInduced Damage in NIH3T3 Cells[J].Journal of Jilin University Science Edition, 2016, 54(06): 1451-1455.

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Protective Effect of 2-TeCD on UVBInduced Damage in NIH3T3 Cells

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