Abstract: The orthogonal experiments were carried out with 3 single factors of time, temperature and ratio of material to liquid. The optimum conditions for the extraction of flavonoids from Pueraria lobata were m(material)∶V(liquid)=(1∶40)g/mL, 60 ℃ as exaction temperature and 3 h as exaction time. The crude extraction rate of P.lobata was 82%, and the highest extraction rate of flavonoids was 1804%. Using the model organism Caenorhabditis elegans as a model, we investigated the antioxidant effects of mass concentrations of 1,2,5 mg/mL of extraction of flavonoids on C.elegans, respectively. The results show that 1 mg/mL flavonoids extracts are fed to C.elegans and cultured 12 h at 35 ℃, the survival rate of C.elegans is significantly higher than that of the control group, i.e., flavonoids extract has obvious antioxidant effect.

Key words: Pueraria lobata, Caenorhabditis elegans, flavonoid, antioxidant