Abstract: The chiral silicon surface coated with cellulosethree (3,5dimethylphenyl carbamate) was used as stationary phase, the solvent of  V(nhexane)∶V(isopropyl alcohol)=95∶5 was used as mobilephase to  separate  2-oxabicyclo［3,3,0］oct-6-en-3-one. The flow rate  was 08 mL/min, the column temperature was 25 ℃, and detection wavelength was 205 nm. The results show that the resolution is 3.0, the enantiomer can be effectively separated, and the optical purity can be determined by area normalization method.

Key words: 2-oxabicyclo［3,3,0］oct-6-en-3-one, enantiomer, separation, optical purity