Abstract: Mn,Ca,Zn,Fe in Inonotus obliquus were detected by flame atomic absorption spectrometry, and Pb,Cu,Cr were detected by graphite furnace atomic absorption spectroscopy. Microwave digestion was used to obtain total mass ratio of metal elements in I.obliquus. The primary forms of water soluble metal elements were gained by ultrasonic water extraction method, and the secondary  speciation analysis of five metal elements were carried out by Tessier step by step extraction method. The results show that the linearity of regression equation of seven metal elements is good, the correlation coefficients are greater than 099, and the detection limits is 0007—2989 ng/mL. Cu in I.obliquus mainly exists in the form of carbonatebound and residue. Ca,Mn,Zn and Fe elements mainly exist in the form of ion exchange and 
residue, Cr mainly exists in the form of residue, and Pb mainly exists in the form of carbonatebound.

Key words: atomic absorption spectrometry, Inonotus obliquus,  , metal element, total amount, primary speciation analysis, secondary speciation analysis