Abstract: Ultrasonic extraction method was used to extract polysaccharides from Cordyceps militaris Link. The optimal extraction process was determined by response surface method. The components of polysaccharides from Cordyceps militaris Link were detected and identified by high performance liquid chromatography. The antiinflammatory effects of polysaccharides from Cordyceps militaris Link was tested by  using RAW264.7 in vitro cell antiinflammatory model. The results show that the optimal extraction process is the  extraction temperature of 65 ℃, ultrasonic power of 1 800 W, extraction time of 30 h, the extraction rate can reach 3156% under this condition. The monosaccharide components are Ara,Gal,Xyl,Man,Glc and Rha. The polysaccharide from Cordyceps militaris Link has antiinflammatory activity.

Key words: Cordyceps militaris Link, polysaccharide, high performance liquid chromatography(HPLC), antiinflammatory activity