Abstract: Ganoderma lucidum spore powder was used as raw material,  ethanol  with a volume fraction of  70%  as extractant.  We adopted a combination of enzymatic  hydrolysis and ultrasound assisted extraction method,  set different liquid-solid ratios,  ultrasound time,  enzymatic hydrolysis time,  and enzyme dosage  as  four factors for a one-way test and designed a response surface experiment to  determine the optimal extraction method and its influencing factors. The   Ganoderma triterpene were separated and purified by using macroporous resin chromatography. By optimizing the  separation and purification process, the optimal elution resin,   eluent volume fraction,  flow rate of the upper sample solution and the mass ratio of the upper sample solution were determined. The compositional differences of the total  Ganoderma triterpenes were analysed by high performance liquid chromatography (HPLC). Though the pre-experimental analysis, the results show that the enzyme + ultrasound assisted extraction is more efficient compared to the single extraction method. Ethanol is used as an extractant to extract triterpenoids from Ganoderma lucidum can enhance the purity of triterpenoids. Under optimal conditions, the  rapid and accurate determination of the triterpene content can be achieved, providing a theoretical basis for the separation and purification of Ganoderma triterpenes.

Key words: Ganoderma , triterpene,  , extraction process,  , process optimization,  , separation and purification