Abstract: Aiming at the problem of the low sensitivity, poor portability and easy influence by environmental temperature of glucose sensors based on biological enzymes, we developed and designed a high sensitivity flexible glucose sensor, and  prepared flexible glucose sensors with Au electrodes based on polyimide (PI) by using the screen-printing technique. We doped reduced graphene oxide (rGO) to improve electrode conductivity and enhance sensor sensitivity when preparing the glucose oxidase mixed solution. Additionally, we designed a portable detection system that could achieve in situ detection and temperature compensation correction through data processing, thereby reducing the impact of temperature on bioenzyme activity and enabling them to work normally at different environmental temperatures. The experimental results show that the glucose sensor has a detection sensitivity of 58.9 μA/(mmol·cm²) within 0—1.2 mmol/L, a detection limit of 5 μmol/L, and exhibits good selectivity, making it suitable for monitoring glucose concentration in human sweat.

Key words: screen-printing, glucose sensor, reduced graphene oxide, detection system